Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Phycoerythrin Conjugated

SKU: orb347513

Description

Mouse IgG (gamma 1, 2a, 2b and 3 chain) antibody (RPE)

Images & Validation

• Tested Applications: DOT, FC, IF
• Dilution Range: FC: 1:100 - 1:250, IF: 1:100 - 1:250
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG subclass pan reactive Phycoerythrin Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Mouse IgG subclass pan reactive Secondary Antibody was produced by repeated immunization with highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
• Purity: Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Conjugation: RPE

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody phycoerythrin conjugation, rabbit anti-Mouse IgG PE conjugated Antibody