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Catalog Number | orb1935433 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB34786 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Human, Rat |
Reactivity | Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 46710 Da |
Target | This Mouse Gsk3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of mouse Gsk3b. |
UniProt ID | Q9WV60 |
NCBI | NP_062801.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Glycogen synthase kinase-3 beta, GSK-3 beta, Serin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Mouse Gsk3b Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane). This demonstrates the Gsk3b antibody detected the Gsk3b protein (arrow).
Anti-Gsk3b Antibody (N-term) at 1:1000 dilution + mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Mouse Gsk3b Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Mouse Gsk3b Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.