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Catalog Number | orb707135 |
---|---|
Category | Proteins |
Description | Recombinant Mouse CD276 Protein is produced by mammalian expression system and the target gene encoding Val29-Phe244 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CD276 |
Entrez | 102657 |
UniProt ID | Q8VE98 |
Protein Sequence | VEVQVSEDPV VALVDTDATL RCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FTEGRDQGSA YSNRTALFPD LLVQGNASLR LQRVRVTDEG SYTCFVSIQD FDSAAVSLQV AAPYSKPSMT LEPNKDLRPG NMVTITCSSY QGYPEAEVFW KDGQGVPLTG NVTTSQMANE RGLFDVHSVL RVVLGANGTY SCLVRNPVLQ QDAHGSVTIT GQPLTFHHHH HH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | B7H3 protein, CD276 antigen protein, 4Ig-B7-H3 pro Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
25.4 kDa | |
Mouse B7-H3, His Tag (orb383527) is expressed from human 293 cells (HEK293). It contains AA Val 29 - Phe 244 (Accession # NP_598744). |
Unconjugated | |
95% | |
50.1 kDa | |
Mouse B7-H3, Fc Tag (orb348819) is expressed from human 293 cells (HEK293). It contains AA Val 29 - Phe 244 (Accession # NP_598744). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 70-75 kDa after removal of the signal peptide. | |
Mammalian |
> 95% by SDS-PAGE. | |
KMP1730, Recombinant Mouse B7-H3/CD276 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Val29-Phe244) of mouse B7-H3/CD276 (Accession #Q8VE98) fused with a 6×His Tag at the C-terminus. |
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