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Description
Research Area
Images & Validation
−
| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Target | CD27 |
| Reactivity | Mouse |
| Endotoxins | Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | PNSCPDKHYW TGGGLCCRMC EPGTFFVKDC EQDRTAAQCD PCIPGTSFSP DYHTRPHCES CRHCNSGFLI RNCTVTANAE CSCSKNWQCR DQECTECDPP LNPALTRQPS ETPSPQPPPT HLPHGTEKPS WPLHRQLPNS TVYSQRSSHR PLCSSDCIRV DDIEGRMDEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 43 KDa. Observed: 50-60 KDa, reducing conditions
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 19 KDa. Observed: 25-30 KDa, reducing conditions
500 μg, 1 mg, 50 μg, 10 μgRecombinant Mouse CD27 Protein [orb2994283]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
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10 μg, 50 μg, 500 μg, 1 mgCd27 Rabbit Polyclonal Antibody [orb1098031]
ELISA, FC, IHC
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Rabbit
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100 μgHuman CD27 Protein, mFc-His Tag [orb689374]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 65-72 kDa after removal of the signal peptide.
Mammalian
10 μg, 100 μg, 50 μg

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Protocol Information
Mouse CD27 protein (orb707133)
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