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Catalog Number | orb348802 |
---|---|
Category | Proteins |
Description | Mouse BTLA, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 30 - Gly 176 (Accession # Q32MV9). |
Reactivity | Mouse |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 18.6 kDa |
Target | BTLA |
UniProt ID | Q32MV9 |
Protein Sequence | Glu 30 - Gly 176 |
Expression System | HEK293 |
Biological Origin | Mouse |
Expression Region | Glu 30 - Gly 176 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.6 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse BTLA protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 41.3 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 17.4 kDa after removal of the signal peptide.The apparent molecular mass of Mouse-BTLA-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
98.00% | |
21.54 kDa (predicted); 33.7 kDa (reducing conditions) |
Greater than 95.0% as determined by SDS-PAGE. | |
HEK293 Cells |