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Catalog Number | orb383532 |
---|---|
Category | Proteins |
Description | Mouse B7-1, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 38 - Lys 245 (Accession # Q00609-1). |
Reactivity | Mouse |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 25.6 kDa |
Target | B7-1, CD80 |
UniProt ID | Q00609 |
Protein Sequence | Val 38 - Lys 245 |
Source | Mouse B7-1, His Tag (orb383532) is expressed from human 293 cells (HEK293). It contains AA Val 38 - Lys 245 (Accession # Q00609-1). |
Expression System | HEK293 |
Biological Origin | Mouse |
Expression Region | Val 38 - Lys 245 |
Endotoxins | 1.0 EU per μg |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.6 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse B7-1 protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation. | |
E.coli |
98.00% | |
26.94 kDa (predicted); 56.66 kDa (reducing conditions) |
98.00% | |
25.1 kDa (predicted); 45-55 kDa (reducing condition, due to glycosylation) |
> 92% by SDS-PAGE. | |
Recombinant Mouse B7-1/CD80 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Val38-Lys245) of mouse B7-1/CD80 (Accession #NP_033985.3) fused with a 6��His Tag at the C-terminus. |
> 97% by SDS-PAGE. | |
Recombinant Mouse B7-1/CD80 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Val38-Lys245) of mouse B7-1/CD80 (Accession #NP_033985.3) fused with a Fc, 6��His Tag at the C-terminus. |