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Catalog Number | orb257961 |
---|---|
Category | Proteins |
Description | Mouse B7-1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Val 38 - Lys 245 (Accession # Q00609-1). |
Reactivity | Mouse |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 50.3 kDa |
Target | B7-1, CD80 |
UniProt ID | Q00609 |
Protein Sequence | Val 38 - Lys 245 |
Source | Mouse B7-1, Fc Tag (orb257961) is expressed from human 293 cells (HEK293). It contains AA Val 38 - Lys 245 (Accession # Q00609-1). |
Expression System | HEK293 |
Biological Origin | Mouse |
Expression Region | Val 38 - Lys 245 |
Endotoxins | 1.0 EU per μg |
Storage | -20℃ |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.3 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse B7-1 protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation. | |
E.coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 49.8 kDa after removal of the signal peptide. The apparent molecular mass of mB7-1-hFc is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
95% | |
50.9 kDa | |
Human B7-1, Mouse IgG2a Fc Tag (orb420077) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1). |