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Mouse Apolipoprotein E protein

SKU: orb391251

Description

Recombinant mouse Apolipoprotein E protein is produced by our mammalian expression system and the target gene encoding Glu19-Gln311 is expressed with a 6His tag at the C-terminus.

Research Area

Metabolism Research

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
TargetAPOE
ReactivityMouse
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceEGEPEVTDQL EWQSNQPWEQ ALNRFWDYLR WVQTLSDQVQ EELQSSQVTQ ELTALMEDTM TEVKAYKKEL EEQLGPVAEE TRARLGKEVQ AAQARLGADM EDLRNRLGQY RNEVHTMLGQ STEEIRARLS THLRKMRKRL MRDAEDLQKR LAVYKAGARE GAERGVSAIR ERLGPLVEQG RQRTANLGAG AAQPLRDRAQ AFGDRIRGRL EEVGNQARDR LEEVREHMEE VRSKMEEQTQ QIRLQAEIFQ ARLKGWFEPI VEDMHRQWAN LMEKIQASVA TNPIITPVAQ ENQVDHHHHH H

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Apolipoprotein E; Apo-E

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Datasheet
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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Mouse Apolipoprotein E protein (orb391251)

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Available Sizes

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10 μg
$ 290.00
50 μg
$ 690.00
500 μg
$ 2,660.00
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