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Catalog Number | orb2321190 |
---|---|
Category | Proteins |
Description | Monkeypox virus (Democratic Republic of the Congo) M1R Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Gly 183 (Accession # EPI_ISL_19093812, GISAID). |
Target | M1R (MPXV) |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Virus |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
UniProt ID | EPI_ISL_19093812 |
MW | 21.2 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.2 kDa. The protein migrates as 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Expression Region | Gly 2 - Gly 183 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
> 90% as determined by SDS-PAGE. |
98.00% | |
20.41 kDa (predicted). Due to glycosylation, the protein migrates to 23-38 kDa based on Tris-Bis PAGE result. |
98.00% | |
21.59 kDa (predicted); 36.52, 29.25, 25.76 and 22.60 kDa (reducing condition, due to glycosylation) |