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| Catalog Number | orb2321167 |
|---|---|
| Category | Proteins |
| Description | Monkeypox virus (Democratic Republic of the Congo) E8L Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 275 (Accession # EPI_ISL_19093812, GISAID). |
| Target | E8L (MPXV) |
| Conjugation | Unconjugated |
| Tag | C-10×His |
| Reactivity | Virus |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| UniProt ID | EPI_ISL_19093812 |
| MW | 33.7 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.7 kDa. The protein migrates as 38-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Expression Region | Met 1 - Thr 275 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Infectious Disease & Virology |
| Note | For research use only |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 32.7 kDa after removal of the signal peptide. The apparent molecular mass of Monkeypox virus E8L-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
>90% as determined by SDS-PAGE | |
33.9 kDa |
>90% as determined by SDS-PAGE | |
33.9 kDa |
>90% as determined by SDS-PAGE. |
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