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Catalog Number | orb2321167 |
---|---|
Category | Proteins |
Description | Monkeypox virus (Democratic Republic of the Congo) E8L Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 275 (Accession # EPI_ISL_19093812, GISAID). |
Reactivity | Virus |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 33.7 kDa |
Target | E8L (MPXV) |
UniProt ID | EPI_ISL_19093812 |
Expression System | HEK293 |
Expression Region | Met 1 - Thr 275 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.7 kDa. The protein migrates as 38-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 32.7 kDa after removal of the signal peptide. The apparent molecular mass of Monkeypox virus E8L-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
98.00% | |
35.75 kDa (predicted); 67.64 kDa and 37.35 kDa (reducing conditions) |
> 90% as determined by SDS-PAGE. |