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Catalog Number | orb2321168 |
---|---|
Category | Proteins |
Description | Monkeypox virus (Democratic Republic of the Congo) A29L Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Glu 110 (Accession # EPI_ISL_19093812, GISAID). |
Target | A29L (MPXV) |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Virus |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
UniProt ID | EPI_ISL_19093812 |
MW | 14.4 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.4 kDa. The protein migrates as 16 kDa,18-20 kDa and 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Expression Region | Met 1 - Glu 110 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 13.4 kDa after removal of the signal peptide. The apparent molecular mass of Monkeypox virus A29L-His is approximately 10-25 kDa due to glycosylation. | |
Mammalian |