Monkey IgM Antibody

SKU: orb346440

Description

Monkey IgM Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Monkey IgM whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Key Properties

• Source: Monkey
• Biological Origin: Monkey
• Isotype: IgM
• Purity: Monkey IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Monkey IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Monkey Serum and anti-Monkey IgM (µ chain specific). No reaction was observed against anti-Monkey IgG F(c). Some light chain cross reactivity will occur with anti-Monkey IgG.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Monkey Immunoglobulin M

Humoral immune response. Plasmas were analyzed before vaccination (week 0, pre-immune) and at weeks 1, 2, 4 and 6 post-vaccination for anti-VSV antibodies. Neutralizing antibody (NA) titer (left panel) was defined as the dilution of serum that inhibited infection of mouse EL4 cells with M51R-eGFP by 50%. IgM [p/n orb346440] (middle panel) and IgG [p/n orb2652733] (right panel) levels are expressed in ng/mL (mean ± SD, triplicate values), measured by ELISA with extrapolation from standard curves. Data for low dose (a) and high dose (b) vaccinations with M51R and M51R-F are shown. Note scale differences for low and high dose data. Statistical significance was determined by two-factor analysis of variance with cohort and time as the two factors. For all three antibody types, statistical significance of p < 0.05 (after correction for multiple comparisons) was obtained for comparisons of cohort 1 versus cohort 3 (low dose M51R vs. M51R-F), cohort 1 versus cohort 2 (low dose M51R vs. high dose M51R), and cohort 3 versus cohort 4 (low dose M51R-F vs. high dose M51R-F), but not for cohort 2 versus cohort 4 (high dose M51R vs. high dose M51R-F). The data shown represent 1 of 2 analyses with similar results performed on plasma from each animal.

SDS-PAGE of Monkey IgM Whole Molecule. Lane 1: Monkey IgM, Non-Reduced. Lane 2: Monkey IgM, Reduced. Load: 1.0 µg per lane. Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.

Westcott, Marlena M. et al. Immunogenicity in African Green Monkeys of M Protein Mutant Vesicular Stomatitis Virus Vectors and Contribution of Vector-Encoded Flagellin Vaccines (Basel), 6, E16 (2018)

PubMed: 29562688