ML-7 hydrochloride

SKU: orb1304588

Description

ML-7 hydrochloride is a cell-permeable, ATP-competitive inhibitor of myosin light chain kinase (MLCK) with a Ki of 300 nM. It is used in vitro and in vivo to investigate cytoskeletal regulation, smooth muscle contraction, and endothelial barrier function in cardiovascular and cell migration studies.

Research Area

Cell Biology, Epigenetics & Chromatin, Metabolism Research, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 110448-33-4
• MW: 452.74
• Purity: 99.00% (May vary between batches)
• Formula: C₁₅H₁₈ClIN₂O₂S
• SMILES: Cl.Ic1cccc2c(cccc12)S(=O)(=O)N1CCCNCC1
• Target: YAP,Serine/threonin kinase,Myosin,PKC,PKA
• Solubility: DMSO:46 mg/mL (101.6 mM)

Bioactivity

• Target IC50:
  PKC:42 μM|PKA:21 μM|MLCK:0.3 μM(Ki)
• In Vivo:
  ML7 is able to improve Vascular endothelial dysfunction(VED) and atherosclerosis(AS) by regulating the expression of the tight junction (TJ) proteins zona occludens (ZO)-1 and occludin via mechanisms involving MLCK and MLC phosphorylation in high-fat diet-fed rabbits. ML7 decreases the expression of MLCK and MLC phosphorylation in the arterial wall of rabbits fed a high-fat diet and reduces lipid deposition lesions in AS rabbits.
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• In Vitro:
  Inhibition of MLCK by ML-7 induces activation of caspase-3 in adherent MCF-10A and MCF-10A Ras-transformed cells. Its treatment results in a dose-dependent decrease in MLC20 phosphorylation and a corresponding increase in cell death of SMC(smooth muscle cells). The inhibitory effect of ML-7 on MLCK is highly selective. The Ki of ML-7 for MLCK is 0.3 μM, while its Ki for protein kinase A is 21 μM and for protein kinase C is 42 μM. Overexpressing Bcl-2 can protect cells against apoptosis induced by ML-7.
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• Cell Research:
  Cells are treated with vehicle alone, Blebbistatin, ML-7, ML-9, latrunculin A or Y-27632 in media without serum at varying concentrations and time points before being fixed or prepared for FACS analysis. Untreated cells are also serum starved for the duration of the inhibitor treatment. Stock solutions of each agent are made in DMSO (50 μM Blebbistatin, 5 mM LA), 50% ethanol (10 mM ML-7), 70% ethanol (20 mM ML-9) or water (20 mM Y-27632) and are maintained at -20°C.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ML-7 HCl, ML7 hydrochloride, ML7 Hydrochloride, ML-7 Hydrochloride, MLCK, ML7, ML-7, ML 7, ML 7 hydrochloride, ML 7 Hydrochloride, Inhibitor, inhibit, Myosin, threoninkinase, Serinekinase, threonin kinase, Serine kinase, PKA, PKC, Yes-associated protein, YAP

Compound Identifiers

PubChem CID

9803932