MK-571 sodium

SKU: orb1697424

Description

MK-571 sodium

Research Area

Immunology & Inflammation, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 115103-85-0
• MW: 537.07
• Purity: 99.58%
• Formula: C₂₆H₂₇ClN₂O₃S₂·Na
• SMILES: [Na+].CN(C)C(=O)CCSC(SCCC([O-])=O)c1cccc(\C=C\c2ccc3ccc(Cl)cc3n2)c1
• Target: LTR,P-gp,Leukotriene Receptor,LPL Receptor
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:2 mg/mL (3.72 mM);H2O:< 1 mg/mL (insoluble);DMSO:25.78 mg/mL (48 mM);Ethanol:< 1 mg/mL (insoluble)

Bioactivity

• Target IC50:
  LTD4:0.22 nM (Ki, In guinea pig lung)|LTD4:2.1 nM (Ki, In human lung)
• In Vivo:
  In human lungs, MK-571 inhibited [3H]LTD4 binding (Ki: 2.1±1.8 nM, n=29), whereas in guinea pig lungs the Ki value was 0.22±0.15 nM (n=35). However, its binding activity to [3H]LTC4 was low or absent (IC50: 32 μM, n=1, human; 23±11 μM, n=16, guinea pig).
• In Vitro:
  In human lungs, MK-571 inhibited [3H]LTD4 binding (Ki: 2.1±1.8 nM, n=29), whereas in guinea pig lungs the Ki value was 0.22±0.15 nM (n=35). However, its binding activity to [3H]LTC4 was low or absent (IC50: 32 μM, n=1, human; 23±11 μM, n=16, guinea pig).
• Cell Research:
  Cells were seeded onto 96 well plates at a concentration of 1×103 cells per well and incubated for 72 h at 37 C and 5% CO2 to allow MRP1 messenger RNA suppression to occur. Cells were then treated with either control media or one of three chemotherapy drugs temozolomide (150 µM), vincristine (100 nM), or etoposide (2 µM). Cells were then returned to the incubator for a further 72 h; after which time, Metylthiazol Tetrazolium (MTT) powder in PBS (50µl of 5 mg/ml) was added to each well. Cells were then incubated for a further 4 h after which all solution was removed and dimethyl sulfoxide (DMSO) was added. After 10 min incubation time at 37 C, absorbance was recorded at 570 nm wavelength and data was recorded and analyzed. Small molecule inhibitors MK571 (25 µM) and Reversan (15µM) were added 7 h prior to carrying out further drug treatment (temozolomide, vincristine or etoposide) or assay assessment (media change for proliferation and 2D-migration assays) (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

L660711, L-660711 (sodium salt), L-660711 sodium, L-660711 sodium salt, LAD2, LeukotrieneReceptor, Leukotriene Receptor, human mast cells, MRP1, MRP4, L 660711, MK571, MK-571, MK571 sodium, MK-571 sodium, MK-571 sodium salt, MK 571, MK 571 sodium, CysLTR1, dyspnea, bronchoconstriction, ATP-binding cassette, ABCC1, sphingosine kinase, Sphingolipids, pulmonary hypertension, RBL-2H3 cells, Verlukast sodium

Compound Identifiers

PubChem CID

16760569