METTL4 Antibody (C-term)

SKU: orb1939018

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:2000, WB: 1:1000, IHC-P-Leica: 1:500, FC: 1:25
• Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB28044
• Target: This METTL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-344 amino acids from the C-terminal region of human METTL4.
• Molecular Weight: 54041 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Methyltransferase-like protein 4, 211-, METTL4

All lanes: Anti-METTL4 Antibody (C-term) at 1:1000 dilution. Lane 1: Human liver lysate. Lane 2: U-2OS whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-METTL4 Antibody (C-term) at 1:2000 dilution. Lane 1: Huamn liver lysate. Lane 2: Rat liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Overlay histogram showing U-2 OS cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

This METTL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-344 amino acids from the C-terminal region of human METTL4.

UniProt

Q8N3J2

RefSeq (Protein):NP_073751.3