Metformin

SKU: orb1298860

Description

Metformin

Research Area

Cell Biology, Epigenetics & Chromatin, Signal Transduction

Key Properties

• CAS Number: 657-24-9
• MW: 129.16
• Purity: 99.77%
• Formula: C₄H₁₁N₅
• SMILES: CN(C)C(=N)NC(N)=N
• Target: Mitophagy,AMPK,Autophagy
• Solubility: H2O:100 mg/mL (774.23 mM);DMSO:49 mg/mL (379.37 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (15.48 mM)

Bioactivity

• Target IC50:
  TNF-α:3.35 μM|PANC1 cells viability:20.95 ± 0.98 mM|B-CPAP cells viability:5.329 mM|SARS-CoV-2 (Caco2 cells):1.43 mM|SARS-CoV-2 (Calu3 cells):0.4 mM
• In Vivo:
  METHODS: To model Metformin-induced diarrhea, Metformin (125-500 mg/kg) was administered orally to healthy and diabetic obese db/db C57BL/6J mice twice daily for thirteen days. Results: Metformin at 1000 mg/kg/day significantly increased fecal water content. Although no diarrhea symptoms were observed in healthy C57BL/6J mice the same dose of Metformin induced severe diarrhea in diabetic obese db/db mice. METHODS: To investigat the Protective effect of Metformin in radiation injury, Metformin (200 mg/kg once daily for three days) was administered orally to BAL-/- mice, which were then exposed to 6-8 Gy of gamma radiation. Results: When administered prior to exposure to radiation, Metformin prolonge the survival of mice exposed to 8 Gy-TBI and increase the survival of mice exposed to 6 Gy-TBI. Pretreatment with Metformin attenuated radiation damage.
• In Vitro:
  METHODS: Ovarian cancer cells A2780 and SKOV3 were treated with Metformin (0.001-50 mM) for 24-48 h. Cell viability was assayed usin the MTS RESULTS: Micromolar concentration of Metformin did not statistically reduc the viability o the A2780 or SKOV3 cell lines. At 48 h, millimolar concentration resulted in cell death. METHODS: Human colorectal cancer cells HCT29 were treated with Metformin (0.6 mM) for 90 h. Cell motility was detected usin the wound healing assay and chamber invasion assay. Results: Metformin inhibite the migration and invasion of HCT29 cells, and Metformin decrease the motility of tumor cells.

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

diabetes, chain, AMPK, Autophagy, barrier, blood-brain, AMP-activated protein kinase, 1,1-Dimethylbiguanide, inhibit, liver, Mitophagy, mitochondrial, Mitochondrial Autophagy, Metformin, insulin, Inhibitor, sensitivity, respiratory, type

Compound Identifiers

PubChem CID

152743144