Metallothionein Antibody

SKU: orb55575

Description

Rabbit Anti-Pseudomonas aeruginosa Metallothionein Polyclonal

Research Area

Epigenetics & Chromatin, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB (1:1000)
• Reactivity: Bacteria
• Application Notes:
  A 1:1000 dilution was sufficient for detection of Metallothionein in 15 µg of pseudomonas aeruginosa purified Metallothionein (pmtA-GST) protein by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein
• Target: Metallothionein
• Molecular Weight: 36 kDa
• Purification: Peptide Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Metallothionein, Metallothionein 1A, Metallothionein 1S, Metallothionein IA, Metallothionein II, Metallothionein-1A, Metallothionein-IA, Metallothionein2, Metallothionein 2, Metallothionein 2A, MT1, MT1A, MT1S, MT2, MT2A, MT 1A, MT IA, MT II, MT-1A, MT-IA, MT1A_HUMAN, MGC32848, MTC, pmtA, Probable Metallothionein, CES 1, CES1

Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag. Load: 25 μg. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST).

Quick Database Links

Gene Symbol

Metallothionein

UniProt

Q9I1X5