MLANA Antibody

SKU: orb1252766

Description

MLANA Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 µg/mLWB: 0.5-1.0 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Due to differences in protocols and secondary antibody used, the Melan-A antibody may require titration for optimal performance.1. FFPE staining is enhanced by boiling sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: A103
• Immunogen: Recombinant human protein was used as the immunogen for the Melan-A antibody.
• Target: MLANA
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1

IHC testing of human melanoma stained with Melan-A antibody (A103). Note cytoplasmic staining of cells.

SDS-PAGE Analysis of Purified, BSA-Free Melan-A Antibody (clone A103). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

MLANA

UniProt

Q16655