MEK2 N-Term Antibody FITC

SKU: orb420249

Description

MEK2 antibody (FITC)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:40,000, WB: 1µg/mL
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Anti-MEK 2 FITC Conjugated (MOUSE) Antibody is suitable for use in Western Blotting and ELISA. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 44 kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 19G10.F1.E2
• Immunogen: Anti-MEK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH.
• Purity: This fluorescein conjugated protein A purified mouse monoclonal antibody reacts specifically with human MEK2. Anti-MEK2 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-MEK2 Antibody FITC conjugation, fluorescein conjugated mouse anti-MEK2 Antibody, MAP2K2, MEK, MEK 2, MKK2, PRKMK2, CFC4, MEK-2 Antibody

Western Blot of Anti-MEK2 Antibody. Lane 1: MEK-1 recombinant protein. Lane 2: MEK-2 recombinant protein. Load: 50 ng per lane. Primary Antibody: Anti-MEK2 supernatant clone neat over night at 4°C. Secondary Antibody: Anti-mouse HRP at 1:40000 dilution.

Quick Database Links

UniProt

P36507