Maribavir

SKU: orb1306272

Description

Maribavir is an orally bioavailable benzimidazole riboside that acts as a selective ATP-competitive inhibitor of the CMV UL97 kinase. It is effective against drug-resistant CMV strains and is used in research applications involving in vitro antiviral assays and in vivo models of cytomegalovirus infection.

Research Area

Infectious Disease & Virology, Protein Biochemistry

Key Properties

• CAS Number: 176161-24-3
• MW: 376.24
• Purity: 99.85% (May vary between batches)
• Formula: C₁₅H₁₉Cl₂N₃O₄
• SMILES: CC(C)Nc1nc2cc(Cl)c(Cl)cc2n1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O
• Target: Antifection,Virus Protease,HCV Protease
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.32 mM);DMSO:60 mg/mL (159.47 mM)

Bioactivity

• Target IC50:
  Histone phosphorylation:3 nM
• In Vitro:
  Maribavir effectively inhibits the autophosphorylation of both the wild type and the major Ganciclovir (GCV)-resistant UL97 mutants, showing a mean IC50 of 35 nM. The M460I mutation increases sensitivity to Maribavir, decreasing its IC50 to 4.8 nM. However, a Maribavir-resistant UL97 mutant (L397R) exhibits significantly reduced kinase activity, functioning at approximately 10% of the wild type levels for both GCV kinase and protein kinase activities. Enzyme kinetic studies reveal that Maribavir acts as a competitive inhibitor against ATP, with a Ki of 10 nM. Furthermore, Maribavir inhibits viral replication in a dose-dependent manner, achieving an IC50 of 0.12±0.01 μM as per a multicycle DNA hybridization assay, and strongly inhibits pUL97 protein kinase with a 50% inhibition concentration of 3 nM.
  Read more...
• Cell Research:
  Maribavir (1263W94) is dissolved in DMSO and stored, and then diluted with appropriate media before use. For these studies MRC-5 cells are seeded in 24-well plates at ~5×104 cells/well and grown for 3 days in MEM 8-1-1 to confluence (~1.1×105 cells/well). The cells are infected with AD169 in MEM 2-1-1 at an MOI ranging from 1 to 3 and incubated at 37°C for 90 min to allow viral adsorption. The unadsorbed virus is removed and replaced with 1 mL of MEM 2-1-1. To test the effect of compounds on viral DNA synthesis or maturation, Maribavir, BDCRB, or GCV is added to the medium at the concentrations indicated for each experiment.
  Read more...

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BW1263W94, CMV, Benzimidavir, GW257406X, Maribavir, HCMV, HCVProtease, HCV Protease

Compound Identifiers

PubChem CID

471161