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Catalog Number | orb1937255 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB32125 |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 39392 Da |
Target | This LPAR6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-134 amino acids from the Central region of human LPAR6. |
UniProt ID | P43657 |
NCBI | NP_001155970.1, NP_005758.2, NP_001155969.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Lysophosphatidic acid receptor 6, LPA receptor 6, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-LPAR6 Antibody (Center) at 1:1000 dilution. Lane 1: Mouse heart tissue lysate. Lane 2: Rat heart tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-LPAR6 Antibody (Center) at 1:2000 dilution. Lane 1: Molt-4 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Mouse heart tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
LPAR6 Antibdy (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of LPAR6 Antibdy (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
LPAR6 Antibody (Center) western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the LPAR6 antibody detected the LPAR6 protein (arrow).