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Catalog Number | orb1937513 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB31354 |
Tested applications | FC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 35941 Da |
Target | This LGALS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human LGALS4. |
UniProt ID | P56470 |
NCBI | NP_006140.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Galectin-4, Gal-4, Antigen NY-CO-27, L-36 lactose- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-LGALS4 Antibody (N-term) at 1:1000 dilution + HT-29 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
LGALS4 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the LGALS4 antibody detected the LGALS4 protein (arrow).
LGALS4 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.