Leptin receptor Rabbit pAb, PE conjugated

SKU: orb124732

Description

Rabbit polyclonal antibody against Leptin Receptor conjugated to PE

Images & Validation

• Tested Applications: IF
• Dilution range: IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Leptin receptor
• Target: LEPR
• Molecular Weight: 130kDa
• Purification: Affinity purified by Protein A
• Conjugation: PE

Storage & Handling

• Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
• Form/Appearance: Lyophilized or Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Concentration: - 100 μg (in 100 μl): 1 mg/ml
• Disclaimer: For research use only

Alternative Names

anti CD 295 antibody, anti CD295 antibody, anti LEP R antibody, anti LEP-R antibody, anti LEPR antibody, anti LEPRD antibody, anti LEPROT antibody, anti Leptin receptor antibody, anti OB R antibody, anti OB receptor antibody, anti OB-R antibody, anti obl antibody, anti Obr antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Leptin receptor antibody (orb124732), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Leptin receptor antibody (orb124732), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: MDA-MB-231. Primary Antibody (green line): Rabbit Anti-Leptin receptor antibody (orb124732), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

LEPR