LDN-57444

SKU: orb1307095

Description

LDN-57444 is a competitive and reversible inhibitor of the ubiquitin carboxyl-terminal hydrolase UCH-L1, exhibiting an IC50 of 0.88 µM. This small molecule is utilized in biochemical and cellular research to investigate proteasomal protein degradation pathways, including studies in models of neurodegenerative disease.

Research Area

Cell Biology, Molecular Biology, Protein Biochemistry

Key Properties

• CAS Number: 668467-91-2
• MW: 397.64
• Purity: 97.80%
• Formula: C₁₇H₁₁Cl₃N₂O₃
• SMILES: CC(=O)ON=C1C(=O)N(Cc2cc(Cl)ccc2Cl)c2ccc(Cl)cc12
• Target: DUB,Apoptosis
• Solubility: DMSO:19.9 mg/mL (50.05 mM);10% DMSO+90% Corn Oil:1.5 mg/mL (3.77 mM)

Bioactivity

• Target IC50:
  UCHL1:0.88 μM
• In Vivo:
  LDN-57444 (25-50 μM) reduces cell viability in a dose-dependent manner by inhibiting the activity of the ubiquitin-proteasome system and increasing the levels of highly ubiquitinated proteins, thereby inducing apoptosis.
• In Vitro:
  In vivo, LDN-57444 significantly alters the distribution of synaptic proteins and the morphology of dendritic spines. It leads to a reduction in UCH-L1 activity but does not affect the protease inhibition of cAMP levels.
• Cell Research:
  MTT assay(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UCH-L1, UCH-L3, Apoptosis, DUBs, Deubiquitinase, inhibit, Inhibitor, LDN57444, LDN-57444, LDN 57444

Compound Identifiers

PubChem CID

24906255