Laurdan

SKU: orb1306584

Description

Laurdan

Key Properties

• CAS Number: 74515-25-6
• MW: 353.54
• Purity: 99.02%
• Formula: C₂₄H₃₅NO
• SMILES: CCCCCCCCCCCC(=O)c1ccc2cc(ccc2c1)N(C)C
• Target: Others
• Solubility: 10% DMSO+90% Corn Oil:1 mg/mL (2.83 mM);DMSO:10 mg/mL (28.29 mM)

Bioactivity

• Cell Research:
  Laurdan as a biosensor to monitor changes in membrane water content during NIH3T3 cell migration I. Solution preparation 1. Preparation of storage solution: Dissolve Laurdan in dimethyl sulfoxide (DMSO) to prepare a 1.8 mM stock solution. 2. Preparation of working solution: Dilute the Laurdan stock solution at a dilution of 1:1000. II. Operation steps 1. Culture NIH3T3 cells in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, 5 mL Pen-Strep, and 2.5 mL 1 M HEPES. 2. Seed freshly isolated NIH3T3 cells into 35 mm Mattek glass-bottom culture dishes coated with fibronectin. 3. Incubate the cells with Laurdan at 37°C for 40 min. 4. Observe using an LSM710 META laser scanning microscope. 5. Calibration of the system and phasor plot of FLIM data by measuring fluorescein (pH 11).

Storage & Handling

• Storage: keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, Laurdan, inhibit, 6-Dodecanoyl-2-dimethylaminonaphthalene

Compound Identifiers

PubChem CID

104983