Kynurenine (L)

SKU: orb1302443

Description

L-Kynurenine is an endogenous tryptophan metabolite that acts as an agonist of the aryl hydrocarbon receptor (AHR). This small molecule is used in research to study immunomodulation, such as T-cell suppression, and cancer biology, including glioma cell invasion in vitro.

Research Area

Immunology & Inflammation

Key Properties

• CAS Number: 2922-83-0
• MW: 208.22
• Purity: >98%
• Formula: C₁₀H₁₂N₂O₃
• SMILES: N[C@@H](CC(=O)c1ccccc1N)C(O)=O
• Target: Tryptophan
• Solubility: Soluble in DMSO (up to 2 mg/ml) or in Water (up to 2 mg/ml).

Bioactivity

• Target IC50:
  GABAA (pentobarbital stimulation of [3H]flunitrazepam binding):35.9 ± 14.8 μM|B16 F10 melanoma cells:1.74 mM
• In Vivo:
  METHODS: To study the effect of L-Kynurenine on arteries, L-Kynurenine was administered to the mesenteric arteries (0.3 mM to 1 mM) and aorta (1 mM to 1 mM) of male Wistar rats. RESULTS: L-Kynurenine induced concentration-dependent diastolic responses in the mesenteric arteries and aorta of rats, while the selective Kv7 inhibitor aspirin and the depolarizing agent potassium chloride eliminated this diastolic effect. METHODS: To study the neuroprotective effect of L-Kynurenine, L-Kynurenine (50-300 mg/kg) was intraperitoneally injected into neonatal rats. One hour after intraperitoneal injection, the young rats were exposed to a hypoxic environment for 1.5 hours to construct a hypoxia-ischemia neonatal rat model. L-Kynurenine (100, 200, 3000, 400 mg/kg) was intraperitoneally injected into neonatal rats. One hour after intraperitoneal injection, NMDA was injected to construct the neonatal rat model of NMDA injury. RESULTS: L-Kynurenine demonstrated a dose-dependent significant neuroprotective effect and had a complete protective effect at a dose of 300 mg/kg.
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• In Vitro:
  METHODS: Human corneal endothelial cells (HCEC) were treated with L-Kynurenine (10-40 µM), and apoptosis of HCECs was evaluated by flow cytometry using fluorescent isothiocyanate Annexin-V conjugates (FITC). RESULTS: L-Kynurenine-induced apoptosis slightly increased. METHODS: NK92 MI cells were treated with L-Kynurenine (0-800 μM) for 72 hours. Cell viability was evaluated by trypan blue staining, and flow cytometry analysis or functional detection was performed. RESULTS: L-Kynurenine dose-dependently induced cell growth inhibition and apoptosis.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

β-Anthraniloyl-L-alanine

Compound Identifiers

PubChem CID

161166