L-Kynurenine

SKU: orb1302443

Description

L-Kynurenine is an endogenous tryptophan metabolite that acts as an agonist of the aryl hydrocarbon receptor (AHR). This small molecule is used in research to study immunomodulation, such as T-cell suppression, and cancer biology, including glioma cell invasion in vitro.

Research Area

Immunology & Inflammation, Metabolism Research

Key Properties

• CAS Number: 2922-83-0
• MW: 208.21
• Purity: 99.91% (May vary between batches)
• Formula: C₁₀H₁₂N₂O₃
• SMILES: N[C@@H](CC(=O)c1ccccc1N)C(O)=O
• Target: Endogenous Metabolite,AhR,Aryl Hydrocarbon Receptor
• Solubility: H2O:4 mg/mL (19.21 mM);DMSO:50 mg/mL (240.14 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:1 mg/mL (4.8 mM)

Bioactivity

• Target IC50:
  GABAA (pentobarbital stimulation of [3H]flunitrazepam binding):35.9 ± 14.8 μM|B16 F10 melanoma cells:1.74 mM
• In Vivo:
  METHODS: To study the effect of L-Kynurenine on arteries, L-Kynurenine was administered to the mesenteric arteries (0.3 mM to 1 mM) and aorta (1 mM to 1 mM) of male Wistar rats. RESULTS: L-Kynurenine induced concentration-dependent diastolic responses in the mesenteric arteries and aorta of rats, while the selective Kv7 inhibitor aspirin and the depolarizing agent potassium chloride eliminated this diastolic effect. METHODS: To study the neuroprotective effect of L-Kynurenine, L-Kynurenine (50-300 mg/kg) was intraperitoneally injected into neonatal rats. One hour after intraperitoneal injection, the young rats were exposed to a hypoxic environment for 1.5 hours to construct a hypoxia-ischemia neonatal rat model. L-Kynurenine (100, 200, 3000, 400 mg/kg) was intraperitoneally injected into neonatal rats. One hour after intraperitoneal injection, NMDA was injected to construct the neonatal rat model of NMDA injury. RESULTS: L-Kynurenine demonstrated a dose-dependent significant neuroprotective effect and had a complete protective effect at a dose of 300 mg/kg.
• In Vitro:
  METHODS: Human corneal endothelial cells (HCEC) were treated with L-Kynurenine (10-40 µM), and apoptosis of HCECs was evaluated by flow cytometry using fluorescent isothiocyanate Annexin-V conjugates (FITC). RESULTS: L-Kynurenine-induced apoptosis slightly increased. METHODS: NK92 MI cells were treated with L-Kynurenine (0-800 μM) for 72 hours. Cell viability was evaluated by trypan blue staining, and flow cytometry analysis or functional detection was performed. RESULTS: L-Kynurenine dose-dependently induced cell growth inhibition and apoptosis.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ArylHydrocarbonReceptor, Aryl Hydrocarbon Receptor, (S)-Kynurenine, AhR, EndogenousMetabolite, Endogenous Metabolite, Inhibitor, Human Endogenous Metabolite, L-Kynurenine, LKynurenine, Kynurenine, L Kynurenine, inhibit

Compound Identifiers

PubChem CID

161166