L-Homocysteine thiolactone hydrochloride

SKU: orb1706153

Description

L-Homocysteine thiolactone hydrochloride is a neurotoxic metabolite implicated in models of excitotoxicity and neurodegeneration. It is used in research to study mechanisms underlying epilepsy and Alzheimer's disease pathology in both cellular and animal model systems.

Research Area

Metabolism Research

Key Properties

• CAS Number: 31828-68-9
• MW: 153.63
• Purity: 99.32% (May vary between batches)
• Formula: C₄H₈ClNOS
• SMILES: N[C@@H]1C(=O)SCC1.Cl
• Target: Amino Acids and Derivatives,Others
• Solubility: H2O:20 mg/mL (130.18 mM);DMSO:100 mg/mL (650.91 mM)

Bioactivity

• In Vivo:
  L-Homocysteine thiolactone hydrochloride dissolved in PBS was injected i.p., into 4-12-week-old mice (40-600 nmol/g body weight). To determine how Pon1 affects its turnover in vivo, L-Homocysteine thiolactone hydrochloride was injected i.p. into Pon1−/− and Pon1+/+ mice, and Hcy-thiolactone kinetics in plasma were monitored. A non-toxic dose of 600 nmol L-Homocysteine thiolactone hydrochloride /g body weight in most experiments, but similar results were obtained with doses as low as 40 nmol/g body weight. This shows that in Pon1−/− mice Hcy-thiolactone metabolism is not impaired during its transit from the intraperitoneal cavity to the bloodstream and suggests that Pon1 has a negligible contribution to Hcy-thiolactone turnover in membranes surrounding the intraperitoneal cavity.
• In Vitro:
  At a concentration of 0.25 mM and after 24 h incubation with L-Homocysteine thiolactone hydrochloride showed around 40% of cell death. Using 1 mM of L-Homocysteine thiolactone hydrochloride and after 24 h incubation up to 80% of cell death was detected. HUVEC cells were therefore incubated with increasing concentrations of Hcy (1 mM–10 mM) or L-Homocysteine thiolactone hydrochloride (0.25 mM–1 mM) for different times. None of the Hcy or L-Homocysteine thiolactone hydrochloride concentrations tested had an effect on TNF-α or IL-1α secretion. However, Hcy or L-Homocysteine thiolactone hydrochloride promoted the release of IL-8 starting from 3 h and increasing according to a dose-time dependent manner.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LHomocysteine thiolactone hydrochloride, L-Homocysteine thiolactone Hydrochloride, L Homocysteine thiolactone hydrochloride

Compound Identifiers

PubChem CID

10313226