KPNA2 Antibody

• Catalog Number: orb1259089
• Category: Antibodies
• Description: KPNA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human KPNA2 (NP_002257.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 55kDa
• Target: KPNA2
• UniProt ID: P52292
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: KPNA2 Antibody: QIP2, RCH1, IPOA1, SRP1alpha, SRP1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using KPNA2 antibody (orb1259089) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of MCF-7 cells using KPNA2 antibody (orb1259089). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of U-251MG cells, using 3 ug KPNA2 antibody (orb1259089). Western blot was performed from the immunoprecipitate using KPNA2 antibody (orb1259089) at a dilution of 1:1000.