Ki67 Antibody

SKU: orb534169

Description

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. [UniProt]

Research Area

Epigenetics

Images & Validation

• Tested Applications: FACS, IF, IHC-P
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting points. Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: PMKI67-1
• Immunogen: A portion of amino acids 2293-2478 was used as the immunogen for the Ki67 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human tonsil stained with Ki67 antibody (clone PMKI67-1). Required HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of human HeLa cells with Ki67 antibody (green, clone PMKI67-1) and Phalloidin (red, membrane stain).

FACS testing of human HeLa cells with Ki67 antibody (blue, clone PMKI67-1) and isotype control (red). Cells were trypsinized and 2-4% PFA-fixed prior to staining.

Quick Database Links

UniProt

P46013