Kanamycin

SKU: orb2813903

Description

Kanamycin A is an orally available aminoglycoside antibiotic that inhibits bacterial protein synthesis by targeting ribosomal RNA. It is widely used in research applications, including in vitro studies against Mycobacterium tuberculosis and in vivo models of bacterial pneumonia.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 8063-07-8
• MW: 484.50
• Purity: 99.96% (May vary between batches)
• Formula: C₁₈H₃₆N₄O₁₁
• SMILES: N[C@@H]1[C@@H](O[C@@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](CN)O2)[C@H](O)[C@@H](O[C@@H]3[C@H](O)[C@@H](N)[C@H](O)[C@@H](CO)O3)[C@H](N)C1
• Target: Antibiotic,Antibacterial
• Solubility: H2O:100 mg/mL (206.4 mM);DMSO:< 1 mg/mL (insoluble)

Bioactivity

• In Vivo:
  The neurons damage of the DCN induced by kanamycin (500 mg/kg/day) is reversible and autophagy is upregulated in the neurotoxic course of kanamycin on DCN through JNK1-mediated phosphorylation of Bcl-2 pathway in rats. The serum BUN and Cr levels are both increased at the 1st day after the period of kanamycin administration. The neurons expressing LC3 are increased at 1, 7 and 14 days after kanamycin administration in comparison to the control group. Kanamycin treatment results in the increase of autophagy in a time-dependent manner. Kanamycin (5 mg/kg) and sodium ampicillin (10 mg/kg) administered intramuscularly (i.m.) separately, and then together, to five pony mares, and the ampicillin concentration exceeds 5 mg/mL in inflamed synovial fluid for some 2.5 h after injection, and kanamycin concentration exceeds 2 mg/mL for 7 h in the pony.
• In Vitro:
  The concentration of Kanamycin above 0.0025% has a significant inhibition on the growth of B. bifidum while having no influence on the other four probiotics at incubation 12 h or 24 h. The optimum selective concentration of kanamycin in MRS media is 0.005% for selective enumeration of B.bifidum.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Antibiotic,Antibacterial