JNJ0966

SKU: orb1302567

Description

JNJ0966 is a potent and selective small molecule inhibitor of matrix metalloproteinase-9 (MMP-9) proenzyme, exhibiting an IC50 of 440 nM. It is a valuable research tool for studying MMP-9's role in inflammatory, oncological, and neurological diseases in both cellular and animal models.

Research Area

Protein Biochemistry

Key Properties

• CAS Number: 315705-75-0
• MW: 360.45
• Purity: 99.62% (May vary between batches)
• Formula: C₁₆H₁₆N₄O₂S₂
• SMILES: COc1ccccc1Nc1nc(cs1)-c1sc(NC(C)=O)nc1C
• Target: MMP
• Solubility: DMSO:150 mg/mL (416.15 mM);10% DMSO+90% Corn Oil:3.3 mg/mL (9.16 mM)

Bioactivity

• Target IC50:
  MMP9 zymogen:440 nM
• In Vivo:
  JNJ0966 demonstrated effectiveness in alleviating the severity of disease in a mouse model of experimental autoimmune encephalomyelitis. Its effectiveness is dose-dependent, showing plasma and brain concentrations of 77.5±31.1 ng/mL (215 nM) and 481.6±162.5 ng/g (~1336 nM) at a 10-mg/kg dose, and 293.6±118.4 ng/mL (815 nM) in plasma and 1394.0±649.1 ng/g (~3867 nM) in the brain at a 30-mg/kg dose, respectively. Moreover, JNJ0966 exhibits a preference for accumulation in the brain, evidenced by brain/plasma ratios of 6.2 at the 10 mg/kg dose and 4.7 at the 30 mg/kg dose.
• In Vitro:
  The activations of proMMP-1, proMMP-2, and proMMP-3 are not significantly different in the presence or absence of 10 μM JNJ0966, whereas proMMP-9 activation by trypsin is significantly attenuated by JNJ0966. The addition of JNJ0966 to the reaction results in a significant reduction in fully processed MMP-9 and an apparent accumulation of the intermediate species.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, JNJ0966, JNJ-0966, JNJ 0966, MMP-9, MMP, Matrix metalloproteinases

Compound Identifiers

PubChem CID

1117189