Ipratropium bromide monohydrate

SKU: orb1224288

Description

Ipratropium Bromide is a muscarinic antagonist, bronchodilator, N-Isopropyl salt of atropine.(In Vitro):Ipratropium bromide hydrate (1 nM, 10 nM, 100 nM; 15 min) exerts its toxic effects via disruption of mitochondrial membrane potential.Ipratropium bromide hydrate (1 nM-1 μM; 4 h) increases infarct size in isolated perfused heart ischaemia/reperfusion experiments with a dose-responsive manner (EC50=22.7 nM).Ipratropium bromide hydrate (0.001 nM-0.1 mM; 2 h) inhibits adult rat cardiac myocyte growth after 4 h hypoxia treatment.(In Vivo):Ipratropium bromide hydrate (1.0 μg/kg; i.v.; single dose) enhances vagal nerve stimulation induing bronchoconstriction.Ipratropium bromide hydrate (0.04 mg/20 mL and 0.20 mg/20 mL; inhalation for 30 min, rate=30 mL/30 min) can protect the lungs against the cadmium-induced acute neutrophilic inflammation by reducing the parenchyma inflammatory infiltration of neutrophils.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 66985-17-9
• MW: 430.38
• Purity: >98% (HPLC)
• Formula: C₂₀H₃₂BrNO₄
• SMILES: CC(C)[N+]1(C)C2CCC1CC(C2)C(=O)C(CO)C1=CC=CC=C1
• Target: AChR
• Solubility: DMSO, Ethanol and Water: 200 mM

Bioactivity

• In Vivo:
  Ipratropium bromide hydrate (1.0 μg/kg; i. v. ; single dose) enhances vagal nerve stimulation induing bronchoconstriction. Ipratropium bromide hydrate (0.04 mg/20 mL and 0.20 mg/20 mL; inhalation for 30 min, rate = 30 mL/30 min) can protect the lungs against the cadmium-induced acute neutrophilic inflammation by reducing the parenchyma inflammatory infiltration of neutrophils. Animal model: Guinea-pigs of the Dunkin Hartley strain. Dosage: 0.1-1 μg/kg. Administration: Intravenous injection; single dose. Result: Resulted little blocking effect on post-junctional muscarinic receptors at 0.3 μg/kg, and inhibited ACh-induced bronchoconstriction at 0.5 μg/kg. Animal model: Male Sprague-Dawley rats (300-350 g). Dosage: 0.04 mg/20 mL and 0.20 mg/20 mL. Administration: Inhalation; atomization rate of 30 mL/30 min; 30 min. Result: Had no significant effects on any parameters recorded in healthy rats but exerted a protective effect against the inflammatory reaction elicited by cadmium.
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• In Vitro:
  Ipratropium bromide hydrate (1 nM, 10 nM, 100 nM; 15 min) exerts its toxic effects via disruption of mitochondrial membrane potential. Ipratropium bromide hydrate (1 nM-1 μM; 4 h) increases infarct size in isolated perfused heart ischaemia/reperfusion experiments with a dose-responsive manner (EC50 = 22.7 nM). Ipratropium bromide hydrate (0.001 nM-0.1 mM; 2 h) inhibits adult rat cardiac myocyte growth after 4 h hypoxia treatment. Cell Viability Assay Cell line: Adult Rat Cardiac Myocyte. Concentration: 0.001 nM-0.1 mM Incubation time: 2 h in dark; prior to 4 h hypoxia. Result: Resulted cell viability in a dose-dependent manner, with the inhibition rate of 52.7% at 0.1 mM dose.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

AChR