Integrin beta 3 Antibody / CD61 / ITGB3

SKU: orb2640000

Description

Reacts with human Integrin beta 3, also called CD61. It associates with the αV-chain (CD51) to form vitronectin receptor, or with the αIIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (Frozen & FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Titration of the Integrin beta 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: ITGB3/1713
• Immunogen: Human recombinant protein used as the immunogen for the Integrin beta 3 antibody.
• Purification: Protein G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human spleen tissue with Integrin beta 3 antibody (clone ITGB3/1713). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

Quick Database Links

UniProt

P05106