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Integrin alpha E Antibody / CD103

Catalog Number: orb699538

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb699538
CategoryAntibodies
DescriptionThis MAb recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).
Species/HostRabbit
ClonalityRecombinant
Clone NumberITGAE/3904R
Tested applicationsIHC-P
ReactivityHuman
IsotypeRabbit IgG
ImmunogenRecombinant human ITGAE partial protein was used as the immunogen for the Integrin alpha E antibody.
Antibody TypePrimary Antibody
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
PurityProtein A affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis recombinant Integrin alpha E antibody is available for research use only.
UniProt IDP38570
StorageStore the recombinant Integrin alpha E antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the recombinant Integrin alpha E antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
Integrin alpha E Antibody / CD103

IHC staining of FFPE human tonsil with recombinant Integrin alpha E antibody (clone ITGAE/3904R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Integrin alpha E Antibody / CD103

SDS-PAGE analysis of purified, BSA-free recombinant Integrin alpha E antibody (clone ITGAE/3904R) as confirmation of integrity and purity.

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    IHC-P

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    Mouse

    Recombinant

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    20 μg