ING5 Antibody

• Catalog Number: orb1256486
• Category: Antibodies
• Description: ING5 Antibody
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ING5 (NP_115705.2).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 28kDa
• Target: ING5
• UniProt ID: Q8WYH8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Inhibitor of growth protein 5, p28ING5, ING5
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ING5 antibody (orb1256486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunofluorescence analysis of MCF-7 cells using ING5 antibody (orb1256486). Blue: DAPI for nuclear staining.