Importazole

SKU: orb1304195

Description

Importazole

Research Area

Cell Biology

Key Properties

• CAS Number: 662163-81-7
• MW: 318.42
• Purity: 99.90%
• Formula: C₂₀H₂₂N₄
• SMILES: CC(Nc1nc(nc2ccccc12)N1CCCC1)c1ccccc1
• Target: Apoptosis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.28 mM);DMSO:29.41 mg/mL (92.36 mM);Ethanol:28 mg/mL (87.93 mM)

Bioactivity

• In Vitro:
  Importazole is an effective inhibitor o the Ran/importin-β interaction in vitro Importazole specifically blocks importin-β-mediated nuclear import both in Xenopus egg extracts and cultured cells, without disrupting transportin-mediated nuclear import or CRM1-mediated nuclear export. When added during mitosis, importazole impair the release of an importin-β cargo FRET probe and causes both predicted and novel defects in spindle assembl. It also inhibits proliferation and induces apoptosis of multiple myeloma cells by blockin the NF-κB signal pathway in vitr.
• Cell Research:
  For All import and export experiments, HEK 293 cells stably expressing NFAT-GFP are grown on glass coverslips to approximately 50% confluency prior to drug treatment. In All cases, importazole is used at 40 μM and leptomycin B is used at 10 ng/mL. For controls, DMSO is used at a concentration of 0.4%. Ionomycin is added at 1.25 μM. Importazole and leptomycin B treatments are All for 1 hour. In All experiments cells are fixed with 4% formaldehyde prior to fluorescence microscopy. DNA is visualized with 1 μg mL Hoechst dye. For quantification, 100 cells from Each condition are analyzed an the percentage that shows nuclear accumulation of NFAT-GFP calculated.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Apoptosis, nuclear transport receptor importin-β, inhibit, Importazole, Inhibitor

Compound Identifiers

PubChem CID

2949965