Importazole

SKU: orb1304195

Description

Importazole is a cell-permeable small molecule that selectively inhibits importin-β, a key nuclear transport receptor. It is widely used in cell biology research to study nucleocytoplasmic transport, protein localization, and cell cycle progression in both in vitro and in vivo experimental models.

Research Area

Cell Biology

Key Properties

• CAS Number: 662163-81-7
• MW: 318.42
• Purity: >99.99% (May vary between batches)
• Formula: C₂₀H₂₂N₄
• SMILES: CC(Nc1nc(nc2ccccc12)N1CCCC1)c1ccccc1
• Target: Apoptosis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.28 mM);DMSO:29.41 mg/mL (92.36 mM);Ethanol:28 mg/mL (87.93 mM)

Bioactivity

• In Vitro:
  Importazole is an effective inhibitor of the Ran/importin-β interaction in vitro. Importazole specifically blocks importin-β-mediated nuclear import both in Xenopus egg extracts and cultured cells, without disrupting transportin-mediated nuclear import or CRM1-mediated nuclear export. When added during mitosis, importazole impairs the release of an importin-β cargo FRET probe and causes both predicted and novel defects in spindle assembly. It also inhibits proliferation and induces apoptosis of multiple myeloma cells by blocking the NF-κB signal pathway in vitro.
• Cell Research:
  For all import and export experiments, HEK 293 cells stably expressing NFAT-GFP are grown on glass coverslips to approximately 50% confluency prior to drug treatment. In all cases, importazole is used at 40 μM and leptomycin B is used at 10 ng/ml. For controls, DMSO is used at a concentration of 0.4%. Ionomycin is added at 1.25 μM. Importazole and leptomycin B treatments are all for 1 hour. In all experiments cells are fixed with 4% formaldehyde prior to fluorescence microscopy. DNA is visualized with 1 μg/ml Hoechst dye. For quantification, 100 cells from each condition are analyzed and the percentage that shows nuclear accumulation of NFAT-GFP calculated.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, nuclear transport receptor importin-β, inhibit, Importazole, Inhibitor

Compound Identifiers

PubChem CID

2949965