IL-4R Rabbit Polyclonal Antibody

• Catalog Number: orb704477
• Category: Antibodies
• Description: IL-4R Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Other
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IL-4R (71-170/825aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 88 kDa
• Target: IL4R
• UniProt ID: P24394
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD124; IL 4R alpha; IL-4 receptor subunit alpha; I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: 293T (Human) Cell Lysate at 30 ug, Lane 3: Raji (Human) Cell Lysate at 30 ug, Lane 4: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-IL-4R (orb704477) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 140-160 kD, Observed band size: 150 kD.

Sample: Lane 1: IL-4R-His protein (1-232) Overexpression 293T (Human) Cell Lysate at 4 ug Lane 2: IL-4R-His protein (1-232) Overexpression 293T (Human) Cell Lysate at 4 ug Primary: Lane 1: Anti-IL-4R (orb704477) at 1/1000 dilution Lane 2: Anti-His tag at 1/1000 dilution Secondary: Lane 1: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Lane 2: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 43-48 kD Observed band size: 43-48 kD.