IL-3 Antibody Peroxidase Conjugated

SKU: orb345165

Description

IL-3 antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
• Reactivity: Human
• Application Notes:
  This IL-3 antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.
• Purity: Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-3 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Conjugation: HRP

Storage & Handling

• Storage: Store Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-IL-3 peroxidase antibody, rabbit anti-Interleukin 3 peroxidase antibody, IL3, interleukin-3, Burst promoting activity, Colony stimulating factor, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor

Western Blot results of Rabbit Anti-IL-3 Peroxidase Conjugated Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: IL-3. Load: 50 ng. Primary Antibody: Rabbit Anti-IL-3 at 1 µg/ml overnight at 4°C. Secondary Antibody: none. Blocking: BlockOut (p/n orb348644) for 30 min at RT.

Quick Database Links

UniProt

P08700

RefSeq (Protein):NP_000579