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IKK beta Antibody
SKU: orb1928598
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 86564 Da |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−IKKB
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Western blot analysis of anti-IKK beta Antibody in Hela cell line lysates (35 ug/lane). IKK beta (arrow) was detected using the purified Pa).
Confocal immunofluorescent analysis of IKK beta Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with IKK beta antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
IKK beta Antibody (orb1928598)
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