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IFT20 Polyclonal Antibody
SKU: orb1419593
Description
Images & Validation
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| Tested Applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Reactivity | Porcine, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Intraflagellar transport protein 20 homolog, hIFT20, IFT20
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Blank control: Mouse spleen.Primary Antibody (green line): Rabbit Anti-IFT20 antibody.Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488. Dilution: 1 µg /test.Protocol. The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IFT20 Polyclonal Antibody (orb1419593)
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