Hygromycin B

SKU: orb1310027

Description

Hygromycin B

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 31282-04-9
• MW: 527.52
• Purity: 98.00% (May vary between batches)
• Formula: C₂₀H₃₇N₃O₁₃
• SMILES: O[C@H]1C2(O[C@]3([C@@](O2)([C@@H](O)[C@@H](CO)O[C@H]3O[C@H]4[C@H](O)[C@@H](NC)C[C@@H](N)[C@@H]4O)[H])[H])O[C@]([C@H](CO)N)([C@H](O)[C@@H]1O)[H]
• Target: ribosome,Antifungal,Antibiotic,Antibacterial
• Solubility: 10% DMSO+90% Saline:3 mg/mL (5.69 mM);DMSO:240 mg/mL (454.96 mM);H2O:125 mg/mL (236.96 mM);5% DMSO+95% Saline:1.58 mg/mL (3 mM);Saline:50 mg/mL (94.78 mM)

Bioactivity

• Target IC50:
  E. coli:20 μg/mL (MIC)
• In Vivo:
  METHODS: To assay antiviral activity in vivo, Hygromycin B (0-5 μmoL/kg) was administered intraperitoneally to MHV-A59-infected BALB/c mice twice daily for three days. RESULTS: Hygromycin B was able to reduce the levels of viral replication and necrotic liver foci in vivo.
• In Vitro:
  METHODS: Mouse PDAC cells 14387T were transfected with lentiCRISPRv2 hygro lentivirus, and after 48 h, the transfected cells were cultured in new medium containing Hygromycin B (500 μg/mL) for two weeks, and the successful transfected cells were screened. RESULTS: Hygromycin B screened the lentivirally successfully transfected cells. METHODS: E. coli was cultured in medium containing Hygromycin B (0-8 μg/mL) and cell growth was monitored using a Klett-Summerson colorimeter. RESULTS: Hygromycin B reduced the number of viable cells and increased doubling time in a concentration-dependent manner.Hygromycin B inhibited the number of viable cells by 50% at a concentration of 20 μg/mL, and halved the rate of growth at a concentration of 25 μg/mL.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Hygromycin B, Hygrovetine, Inhibitor, Fungal, 70S ribosome, Antibiotic, Bacterial

Compound Identifiers

PubChem CID

23777801