Recombinant Human/Mouse/Rat Activin A Protein

SKU: orb3002480

Description

Recombinant Human Activin A is produced by our Mammalian expression system and the target gene encoding Gly311-Ser426 is expressed.

Key Properties

• Expression System: Human Cells
• Biological Activity: Cell Based Assay: Measured by its ability to induce SMAD signaling in 293-Activin A Res cells(Cat#Xorb2994727). The ED50 for this effect is 1-5 ng/mL.(QC verified). Human intestinal organoids (ipsc-derived) were cultured with Activin A(Cat#orb3002480) , BMP-4(Cat#orb3002515) , EGF (Cat#orb3002493) , Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448), R-spondin 1 (Cat#orb3002497) and FGF-4 (Cat#orb3002445) . The organoids showed good morphology.
• Tag: Tag free
• Expression Region: Gly311-Ser426
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• MW: Predicted: 13 KDa. Observed: 15 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 4mM HCl,4%Mannitol.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibin beta A chain; INHBA; Activin A

Quick Database Links

UniProt

P08476