Human Visfatin 1 protein

SKU: orb707072

Description

Recombinant Human Visfatin Protein is produced by E.coli expression system and the target gene encoding Met1-His491 is expressed with a 6His tag at the N-terminus.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: NAMPT
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MNHKVHHHHH HMNPAAEAEF NILLATDSYK VTHYKQYPPN TSKVYSYFEC REKKTENSKL RKVKYEETVF YGLQYILNKY LKGKVVTKEK IQEAKDVYKE HFQDDVFNEK GWNYILEKYD GHLPIEIKAV PEGFVIPRGN VLFTVENTDP ECYWLTNWIE TILVQSWYPI TVATNSREQK KILAKYLLET SGNLDGLEYK LHDFGYRGVS SQETAGIGAS AHLVNFKGTD TVAGLALIKK YYGTKDPVPG YSVPAAEHST ITAWGKDHEK DAFEHIVTQF SSVPVSVVSD SYDIYNACEK IWGEDLRHLI VSRSTQAPLI IRPDSGNPLD TVLKVLEILG KKFPVTENSK GYKLLPPYLR VIQGDGVDIN TLQEIVEGMK QKMWSIENIA FGSGGGLLQK LTRDLLNCSF KCSYVVTNGL GINVFKDPVA DPNKRSKKGR LSLHRTPAGN FVTLEEGKGD LEEYGQDLLH TVFKNGKVTK SYSFDEIRKN AQLNIELEAA HH

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PBEF; PBEF1; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin

Quick Database Links

Gene Symbol

NAMPT

UniProt

P43490

NCBI Gene ID

10135