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Catalog Number | orb257916 |
---|---|
Category | Proteins |
Description | Human VCAM-1, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Pro 697 (Accession # NP_001069). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 74.9 kDa |
Target | VCAM-1, CD106 |
UniProt ID | P19320 |
Protein Sequence | NP_001069 |
Source | Human VCAM-1, His Tag (orb257916) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Pro 697 (Accession # NP_001069). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Phe 25 - Pro 697 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001069 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | VCAM1,CD106,INCAM-100,V-CAM 1,VCAM-1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 74.9 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CD106 protein
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 75.1 kDa after removal of the signal peptide. The apparent molecular mass of CD106-His is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
98.00% | |
77.36 kDa (predicted); 92.1 kDa (reducing conditions) |
98.00% | |
76 kDa (predicted); 110 kDa (reducing condition, due to glycosylation) |