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Catalog Number | orb429104 |
---|---|
Category | Proteins |
Description | Recombinant of human UCHL3 protein. Ubiquitin carboxyl-terminal hydrolase isozyme L3 belongs to a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to produce the ubiquitin monomer. UCHL3 takes part in the regulation of neuronal development and spermatogenesis and is associated to neurodegenerative diseases. UCHL3 has a 54% homology to UCHL1. |
Tag | 20 amino acid His-tag at N-terminus |
Immunogen | non-glycosylated polypeptide chain containing 250 amino acids (1-230aa) |
Concentration | 1mg/ml |
Form/Appearance | Liquid |
Purity | > 95% as determined by SDS-PAGE |
MW | 28.3kDa |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA |
Source | Escherichia Coli |
Biological Activity | Specific activity: > 3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C |
Storage | Store at 4°C if the entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze thaw cycles. |
Buffer/Preservatives | 20mM Tris-HCl buffer (pH8.0), 1mM DTT, and 10% glycerol. |
Alternative names | Ubiquitin Carboxyl-Terminal Esterase L3 (ubiquitin Read more... |
Note | For research use only |
Application notes | Enzymes |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
27.0 kDa | |
Human UCH-L3, His Tag (orb348793) is expressed from E. coli cells. It contains AA Glu 2 - Ala 230 (Accession # P15374-1). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
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