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Catalog Number | orb392674 |
---|---|
Category | Proteins |
Description | Recombinant human UCHL3 protein is produced by our E. coli expression system and the target gene encoding Met1-Ala230 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.0 |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | UCHL3 |
Entrez | 7347 |
UniProt ID | P15374 |
Protein Sequence | MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.0 |
Alternative names | Ubiquitin carboxyl-terminal hydrolase isozyme L3; Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
27.0 kDa | |
Human UCH-L3, His Tag (orb348793) is expressed from E. coli cells. It contains AA Glu 2 - Ala 230 (Accession # P15374-1). |
> 95% as determined by SDS-PAGE | |
28.3kDa | |
Escherichia Coli |
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