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| Catalog Number | orb429078 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human UBE2I (His) protein |
| Form/Appearance | Sterile Filtered white lyophilized powder. |
| Buffer/Preservatives | Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5. |
| Purity | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Protein Sequence | MHHHHHHAMGTLNMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS |
| Application notes | Enzymes |
| Source | Escherichia Coli |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized UBE2I in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Stability: Lyophilized UBE2I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution UBE2I should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
| Alternative names | SUMO-conjugating enzyme UBC9, EC 6.3.2.-, SUMO-pro Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
>90% as determined by SDS-PAGE. | |
16.73 kDa |
>90% as determined by SDS-PAGE. | |
16.73 kDa |
> 95 % by SDS-PAGE and HPLC analyses. | |
Approximately 19.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids (a.a.) of human UBE2I/UBC9 and 8 a.a. vector sequence including 6 x His tag at N-terminus. |
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