You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb257719 |
---|---|
Category | Proteins |
Description | Human TrkB, His Tag is expressed from human 293 cells (HEK293). It contains AA Cys 32 - His 430 (Accession # AAH31835). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 45.2 kDa |
Target | TrkB, NTRK2 |
UniProt ID | Q16620 |
Protein Sequence | AAH31835 |
Source | Human TrkB, His Tag (orb257719) is expressed from human 293 cells (HEK293). It contains AA Cys 32 - His 430 (Accession # AAH31835). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Cys 32 - His 430 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH31835 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | NTRK2,TRKB,GP145-TrkB Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 45.2 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human TrkB protein
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.0 kDa after removal of the signal peptide. The apparent molecular mass of NTRK2-His is approximately 55-100 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
98% | |
70.4 kDa | |
Human TrkB, Fc Tag (orb257720) is expressed from human 293 cells (HEK293). It contains AA Cys 32 - His 430 (Accession # AAH31835). |
Greater than 95% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
99.30% | |
45.7 kDa (predicted); 70-80 kDa (reducing condition, due to glycosylation) |
> 97% by SDS-PAGE. | |
Recombinant Human TrkB Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Cys32-His Tag430) of human TrkB (Accession #NP_001018074.1) fused with a 6��His Tag at the C-terminus. |