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Catalog Number | orb392588 |
---|---|
Category | Proteins |
Description | Recombinant human TNFRSF11A protein is produced by our mammalian expression system and the target gene encoding Ile30-Pro212 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | TNFRSF11A |
Entrez | 8792 |
UniProt ID | Q9Y6Q6 |
Protein Sequence | IAPPCTSEKH YEHLGRCCNK CEPGKYMSSK CTTTSDSVCL PCGPDEYLDS WNEEDKCLLH KVCDTGKALV AVVAGNSTTP RRCACTAGYH WSQDCECCRR NTECAPGLGA QHPLQLNKDT VCKPCLAGYF SDAFSSTDKC RPWTNCTFLG KRVEHHGTEK SDAVCSSSLP ARKPPNEPHV YLPVDHHHHH H |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | RANK; Tumor necrosis factor receptor superfamily m Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE | |
Unconjugated | |
> 98% by SDS-PAGE and HPLC analyses | |
19.1 kDa | |
E. coli |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 46.2 kDa after removal of the signal peptide. The apparent molecular mass of TNFRSF11A-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
95% | |
47.2 kDa | |
Human RANK, Mouse IgG2a Fc Tag, low endotoxin (orb511285) is expressed from human 293 cells (HEK293). It contains AA Ile 30 - Pro 212 (Accession # Q9Y6Q6-1). |
> 95% by SDS-PAGE. | |
KMP1618, Recombinant Human RANK/TNFRSF11A/CD265 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Ile30-Pro212) of human RANK/TNFRSF11A/CD265 (Accession #Q9Y6Q6) fused with a 6×His Tag at the C-terminus. |
≥90% as determined by SDS-PAGE | |
This protein contains the human TNFRSF11A(Met1-Pro212) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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