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Catalog Number | orb1729592 |
---|---|
Category | Proteins |
Description | Tumor necrosis factor alpha (TNF-α),is a member of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation and immune system development. TNF-α is produced by a wide variety of cell types including lymphoid cells. TNFa matures through separation of the extracellular domain from rest of the molecule. It forms a soluble trimer which binds the trimeric TNF receptor (TNFR). TNF-α is a key cytokine in the development of several inflammatory disorders |
Species/Host | E. coli |
Tested applications | SDS-PAGE |
Reactivity | Human, Mouse |
Tag | Histidine |
Concentration | 1mg/mL |
Dilution range | NA |
Form/Appearance | Lyophilized |
Purity | ≥98% |
Conjugation | Unconjugated |
MW | ~26 kDa |
Hazard Information | For Research use only |
Solubility (25°C) | Soluble in PBS at 25°C |
Protein Sequence | MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREESPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL |
Source | Human |
Expression System | Intercellular |
Biological Origin | Recombinant protein expressed as His tage protein in E.coli |
Endotoxins | Not tested |
Storage | After reconstitution, store at -20°C; Avoid repeated freeze thaw |
Buffer/Preservatives | No preservative |
Alternative names | TNFSF1A, TNFSF2 Read more... |
Note | For research use only |
Application notes | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
17.4 kDa | |
Human TNF-alpha, premium grade (orb257883) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1). |
Unconjugated | |
95% | |
18.3 kDa | |
Human TNF-alpha, His Tag (active trimer) (MALS verified) (orb383503) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide. | |
Mammalian |
Unconjugated | |
90% | |
18.6 kDa | |
Canine TNF-alpha, His Tag (orb762406) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P51742-1). |
Unconjugated | |
95% | |
19.1 kDa | |
Mouse TNF-alpha, His Tag (orb1496307) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804). |